06-04-2026
B.Sc. Nursing Semester-III (MAIN) Examination April 2026 Applied Microbiology and Infection Control Including Safety
Time: Three Hours
Maximum Marks:75
Instructions to the candidates: -
- Do not write anything on question paper except Roll Number, otherwise it shall be deem as an act of indulging in unfair-means and action shall be taken as per rules.
SECTION A (Applied Microbiology -37 Marks)
Q1. Long/Essay type questions (Attempt any one): 1x10=1
- Discuss in detail about types of staining. Explain the principles of microbiology
- Describe about types of Immunity & explain the storage, handling and transportation of vaccine.
Q.2 Short notes (Attempt any three): 3x5=15
- Antigen and Antibody reactions
- National Immunization schedule
- Types of vectors
- Life cycle of malaria Parasite
Q.3 Very Short questions (Attempt any three):3x2=6
- ELISA
- VDRL
- Live Vaccines
- Parasites
Q.4 Answer the following MCQ: 6x1=6
i) Sterlisation of Glass wares is done through
- Dry Heat
- Fumigation
- Moist Heat
- Disinfection
Explanation:
- Glassware (e.g., test tubes, Petri dishes) is sterilized in a hot air oven using dry heat because moisture is not required and glass tolerates high temperatures.
ii ) Sterilization of surgical equipment is done by.
- Autoclave
- Formaldehyde
- Seitz filter
- Radiation
Explanation:
- An autoclave uses moist heat (steam under pressure) at 121°C for 15–20 minutes, which is the standard method for sterilizing surgical instruments and dressings.
ili ) Who is known as the father of microbiology.
- Edwin John butler
- Antony Van leeuwenhock
- Ferdinand cohn
- Robert koch
Explanation:
- He discovered microorganisms using his handmade microscopes and is widely called the Father of Microbiology.
iv) Which the following is not used for the cultivation of virus-
- Tissue culture
- Enbryonated Eggs
- Animal Inoculation
- Enrichment Media
v) Common source of infection for botulism-
- Infected Egg
- Contact with Infectious animal
- Contaminated water
- Bottle packed vegetables
Explanation:
- He discovered microorganisms using his handmade microscopes and is widely called the Father of Microbiology.
vi) Mycology is study of-
- Virus
- Bacteria
- Fungi
- Cell
Explanation-
- Mycology is the branch of microbiology that deals with the study of fungi (yeasts, molds, mushrooms).
SECTION B (Applied Infection Control including safety - 38 Marks)
Q.1 Long/Essay type questions Attempt any one): 1x10=10
- Describe in detail about disinfrection & sterilization methods
- Explain about Hospital acquired infection. Explain preventive measures of UTI.
Q.2 Short notes (Attempt any three): 3x5=15
- BMW
- 5S safety protocols
- Role of nurse in disaster
- Principles of specimen collection.
Q.3 Very Short questions (Attempt any three): 3x2=6
- Types of Hand Hygiene
- Radiation safety
- Needle stick Injury
- WHO
Q.4 Answer the following MCQ: 7x1=7
i) MMR vaccine used for-
- Measles
- Mumps
- Measles, Mumps
- Measles, Mumps, Rubella
ii) Which of following chemical agent is used for Fumigation
- Alcohol
- Iodine
- Formaldehyde
- Carbolic Acid
iii ) BCG Vaccine is scheduled at age of
- Immediate after birth
- At 09 month
- At 06 month
- At 12 month
iv) The most common method of Reproduction of bacteria is-
- Budding
- Binary fission
- Sexual
- Spore formation
v) HIV stands for-
- Human immune virus
- Human immune deficit virus
- Hormonal immune deficiency virus
- Human immunodeficiency virus
vi) Growth and multiplication of micro-organism is known as-
- Isolation
- Autoclave
- Culture
- Stain
vii) Staining technique used in RNTCP-
- PAS
- Gram stain
- Ziehl Neeisin stain
- Albert stain
- Explanation: Used to detect acid-fast bacilli (AFB) in tuberculosis under the Revised National Tuberculosis Control Programme (RNTCP).